常见6种有益生物细菌的培养基配方
2018-07-09 15:08:33 点击:
1. Acetobacter Medium (醋酸菌培养基)
Glucose (葡萄糖) 100g
Yeasst extract (酵母膏) 10g
CaCO3 20g Agar (琼脂) 15g
Distilled water (蒸馏水) 1000ml
Adjust (调) pH to6.8
适用范围:恶臭醋酸杆菌混浊变种
2. Lactic-bacteria Medium I (乳酸菌培养基 I )
Yeast extract (酵母膏) 7.5g
Peptone (蛋白胨) 7.5g
Glucose (葡萄糖) 10g
KH2PO4 2g
Tomato juice (西红柿汁) 100ml
Tween (吐温) 800.5ml
Distilled water (蒸馏水) 900ml pH 7.0
适用范围:植物乳杆菌(胚芽乳杆菌)、嗜热乳酸链球菌
3. Lactic-bacteria Midium Ⅱ (乳酸菌培养基 Ⅱ)
Lacto-casein peptone (乳酪蛋白胨) 10g
Beef extract (蛋白胨)10g
Yeast extract (酵母膏) 5g
Glucose (葡萄糖) 5g
Tween (吐温) 80 1g
K2HPO4 2g
Na-acetate (醋酸钠) 5g
Diamine citrate (柠檬酸二胺)2g
MgSO4.7H2O 0.2g
MnSO4.H2O 0.05g
Distilled water (蒸馏水) 1000m pH 6.5-6.8
适用范围:植物乳杆菌(胚芽乳杆菌)
4.枯草芽孢杆菌常用培养基配方:
1升蒸馏水+20g葡萄糖+15g蛋白胨+5g氯化钠+0.5g牛肉膏+20g琼脂
5.光合细菌培养基配方:
NH4Cl 1.0g
CH3COONa 3.5g
MgCl2 0.1g
CaCl2 0.1g
KH2PO4 0.6g
K2HPO4 0.4g
酵母膏 0.1g
蒸馏水1000mI
pH7.2
固体培养基,由在液体培养基中加入2% 的琼脂制成。
6.酵母菌的培养 配方:
1% Yeast Extract(酵母膏)
2% Peptone(蛋白胨)
2% Dextrose (glucose)(葡萄糖)
若制固体培养基,加入2%琼脂粉
Glucose (葡萄糖) 100g
Yeasst extract (酵母膏) 10g
CaCO3 20g Agar (琼脂) 15g
Distilled water (蒸馏水) 1000ml
Adjust (调) pH to6.8
适用范围:恶臭醋酸杆菌混浊变种
2. Lactic-bacteria Medium I (乳酸菌培养基 I )
Yeast extract (酵母膏) 7.5g
Peptone (蛋白胨) 7.5g
Glucose (葡萄糖) 10g
KH2PO4 2g
Tomato juice (西红柿汁) 100ml
Tween (吐温) 800.5ml
Distilled water (蒸馏水) 900ml pH 7.0
适用范围:植物乳杆菌(胚芽乳杆菌)、嗜热乳酸链球菌
3. Lactic-bacteria Midium Ⅱ (乳酸菌培养基 Ⅱ)
Lacto-casein peptone (乳酪蛋白胨) 10g
Beef extract (蛋白胨)10g
Yeast extract (酵母膏) 5g
Glucose (葡萄糖) 5g
Tween (吐温) 80 1g
K2HPO4 2g
Na-acetate (醋酸钠) 5g
Diamine citrate (柠檬酸二胺)2g
MgSO4.7H2O 0.2g
MnSO4.H2O 0.05g
Distilled water (蒸馏水) 1000m pH 6.5-6.8
适用范围:植物乳杆菌(胚芽乳杆菌)
4.枯草芽孢杆菌常用培养基配方:
1升蒸馏水+20g葡萄糖+15g蛋白胨+5g氯化钠+0.5g牛肉膏+20g琼脂
5.光合细菌培养基配方:
NH4Cl 1.0g
CH3COONa 3.5g
MgCl2 0.1g
CaCl2 0.1g
KH2PO4 0.6g
K2HPO4 0.4g
酵母膏 0.1g
蒸馏水1000mI
pH7.2
固体培养基,由在液体培养基中加入2% 的琼脂制成。
6.酵母菌的培养 配方:
1% Yeast Extract(酵母膏)
2% Peptone(蛋白胨)
2% Dextrose (glucose)(葡萄糖)
若制固体培养基,加入2%琼脂粉
- 上一篇:异位发酵床不分解粪便臭味重?提醒:你的发酵床菌种选对了吗 [2018-07-10]
- 下一篇:想自制EM菌液,初次购买菌种,以后每次都要购买菌种吗? [2018-07-09]